8XTU
Crystal Structure of Rab5b GTPase domain from Leishmania donovani in complex with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-09-06 |
| Detector | DECTRIS EIGER2 X CdTe 16M |
| Wavelength(s) | 0.885 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 39.066, 61.861, 73.635 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.360 - 2.500 |
| R-factor | 0.22038 |
| Rwork | 0.216 |
| R-free | 0.26324 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.409 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.400 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 6617 | 739 |
| <I/σ(I)> | 8.3 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.2 | |
| CC(1/2) | 0.996 | 0.964 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | Sodium Acetate, MES Hydrate pH 6.7, PEG 3350 |
