8XRF
The crystal structure of AsfvTopII in complex with G-DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-06-20 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.987 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 135.770, 126.410, 135.690 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.860 - 2.940 |
R-factor | 0.2199 |
Rwork | 0.218 |
R-free | 0.26260 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.864 |
Data reduction software | XDS |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.19.1_4122: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.860 | 3.020 |
High resolution limit [Å] | 2.940 | 2.940 |
Rmerge | 0.234 | 1.245 |
Rmeas | 0.255 | 1.346 |
Rpim | 0.100 | 0.510 |
Total number of observations | 327604 | 25249 |
Number of reflections | 50298 | 3661 |
<I/σ(I)> | 5.9 | 1.5 |
Completeness [%] | 99.9 | |
Redundancy | 6.5 | 6.9 |
CC(1/2) | 0.981 | 0.609 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298.15 | 40% MPD |