8XQ1

Crystal structure of spleen tyrosine kinase(SYK) in complex with SKI-G-1673

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-11-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9990
Spacegroup name	P 1 21 1
Unit cell lengths	39.632, 85.152, 89.506
Unit cell angles	90.00, 90.02, 90.00

Refinement procedure

Resolution	42.580 - 1.500
R-factor	0.1876
Rwork	0.186
R-free	0.20910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.406
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.530
High resolution limit [Å]	1.500	1.500
Number of reflections	94767	6259
<I/σ(I)>	4.02
Completeness [%]	99.3
Redundancy	5.5
CC(1/2)	0.780	0.780

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M Bis-Tris pH 6.5, 20% PEG 1500