8XPX
The Crystal Structure of PARP12 from Biortus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-10-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 93.940, 74.930, 61.490 |
| Unit cell angles | 90.00, 113.83, 90.00 |
Refinement procedure
| Resolution | 46.600 - 1.750 |
| Rwork | 0.164 |
| R-free | 0.19420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.116 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.600 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.079 | 0.728 |
| Number of reflections | 38860 | 2153 |
| <I/σ(I)> | 10.8 | |
| Completeness [%] | 98.7 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M NH4Ac, 0.1M Na3 Citrate pH5.2, 30% PEG 4000 |
