8XPN
The Crystal Structure of USP8 from Biortus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-11-09 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.95355 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.830, 109.342, 180.992 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.777 - 2.100 |
| Rwork | 0.176 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.250 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.777 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.149 | 0.973 |
| Number of reflections | 67580 | 4460 |
| <I/σ(I)> | 11.7 | |
| Completeness [%] | 99.5 | |
| Redundancy | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 15% PEG 2000 MME, 0.1M Bis-Tris propane pH6.9 |
