8XP4

Crystal Structure of human lysyl-tRNA synthetase with acetyllysine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL18U
Synchrotron site	NFPSS
Beamline	BL18U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97921
Spacegroup name	P 21 21 21
Unit cell lengths	130.337, 90.469, 107.201
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.440 - 2.260
R-factor	0.2397
Rwork	0.239
R-free	0.26350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.576
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.440	2.341
High resolution limit [Å]	2.260	2.260
Rmerge	0.217
Rmeas	0.226
Rpim	0.062
Number of reflections	114081	5822
<I/σ(I)>	7.77
Completeness [%]	99.5
Redundancy	13.4
CC(1/2)	0.994	0.888

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION, RECRYSTALLIZATION		289	0.06 M Morpheus Divalents, 0.1 M Morpheus Buffer system 1, pH 6.5 and 30% Morpheus Precipitant Mix 2