8XNA

Crystal structure of serine hydroxymethyltransferase 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00000
Spacegroup name	P 65 2 2
Unit cell lengths	160.605, 160.605, 210.404
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.380 - 3.800
Rwork	0.198
R-free	0.26430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.236
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.380	4.250
High resolution limit [Å]	3.800	3.800
Number of reflections	16314	4525
<I/σ(I)>	12.9
Completeness [%]	99.6
Redundancy	18.9
CC(1/2)	0.993	0.963

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1 M (NH4) tartrate pH 7.0, 12% PEG3350