8XLD

Structure of the GFP:GFP-nanobody complex from Biortus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-08-12
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.953729
Spacegroup name	I 2 2 2
Unit cell lengths	78.544, 88.363, 122.842
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.221 - 2.100
Rwork	0.162
R-free	0.20920
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.454
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.221	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.166	1.083
Number of reflections	25324	2032
<I/σ(I)>	11.5
Completeness [%]	100.0
Redundancy	13.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M Na2SO4, 0.1M Bis-Tris propane pH6.5, 20% PEG 3350