8XKT

The structure of Spodoptera litura chemosensory protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	CCD
Collection date	2022-06-09
Detector	OXFORD ONYX CCD
Wavelength(s)	1.54
Spacegroup name	P 43 2 2
Unit cell lengths	40.196, 40.196, 133.821
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.280 - 2.500
R-factor	0.2351
Rwork	0.235
R-free	0.24620
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.783
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.300	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.288	0.987
Rmeas	0.313	1.067
Number of reflections	4252	594
<I/σ(I)>	7.4	2.6
Completeness [%]	99.8	100
Redundancy	6.2	6.7
CC(1/2)	0.978	0.659

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	3 M (NH4)2SO4, 0.1 M NaAsO2 (pH 6.5)