8XIE
Archaeal exosome complex (Rrp4-Rrp41-Rrp42)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-16 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 240.822, 240.822, 216.830 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.430 - 3.500 |
| R-factor | 0.2295 |
| Rwork | 0.229 |
| R-free | 0.27310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond angle | 7.083 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20_4459: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.600 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.180 | 0.390 |
| Number of reflections | 86230 | 86210 |
| <I/σ(I)> | 5.3 | 2 |
| Completeness [%] | 94.4 | |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | sodium acetate trihydrate, ammonium sulfate, 20% glycerol, pH 4.9 |
