8XFZ

The structure of HLA-A/L1-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979183
Spacegroup name	P 21 21 21
Unit cell lengths	56.490, 70.826, 117.044
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.240 - 2.320
R-factor	0.2001
Rwork	0.198
R-free	0.25070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.875
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.250	2.400
High resolution limit [Å]	2.320	2.320
Rpim	0.029	0.029
Number of reflections	21060	21060
<I/σ(I)>	18
Completeness [%]	100.0
Redundancy	13.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	0.1M Imidazole, 20%w/v PEG 6000