8XDO
O-methyltransferase from Lycoris longituba complexed with Mg and SAH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-12-19 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.04500 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 49.637, 81.183, 118.027 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.730 - 1.370 |
R-factor | 0.1461 |
Rwork | 0.145 |
R-free | 0.16830 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.320 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.730 | 1.390 |
High resolution limit [Å] | 1.370 | 1.370 |
Rmerge | 0.089 | 1.249 |
Number of reflections | 193630 | 4947 |
<I/σ(I)> | 16.9 | 2.2 |
Completeness [%] | 100.0 | 99.8 |
Redundancy | 13.5 | 13 |
CC(1/2) | 0.999 | 0.797 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 21 % (w/v) PEG 8000, 0.2M MgCl2, 0.1M Tris-HCl (pH 8.0) |