8XAK
Structure of Pif1-G4 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 56.163, 125.141, 258.233 |
| Unit cell angles | 90.00, 91.47, 90.00 |
Refinement procedure
| Resolution | 48.000 - 3.500 |
| R-factor | 0.2859 |
| Rwork | 0.282 |
| R-free | 0.32350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.061 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.17.1_3660: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 3.625 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Number of reflections | 22444 | 2168 |
| <I/σ(I)> | 3.05 | |
| Completeness [%] | 99.3 | |
| Redundancy | 1.9 | |
| CC(1/2) | 0.904 | 0.611 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M MES pH 6.9, 22.5 %(w/v) PEG 400, 0.1 M sodium acetate |
