8XA9
Human MGME1 in complex with 5'-overhang DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2023-06-09 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 180.756, 56.193, 114.445 |
| Unit cell angles | 90.00, 113.02, 90.00 |
Refinement procedure
| Resolution | 24.460 - 2.320 |
| R-factor | 0.259 |
| Rwork | 0.258 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.616 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.400 |
| High resolution limit [Å] | 2.320 | 2.350 |
| Rmeas | 0.066 | 0.608 |
| Rpim | 0.035 | 0.335 |
| Number of reflections | 43737 | 2393 |
| <I/σ(I)> | 13.6 | |
| Completeness [%] | 96.4 | 95.5 |
| Redundancy | 3.3 | 3.1 |
| CC(1/2) | 0.925 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 283 | PEG 3350, sodium citrate/citric acid, sodium tribasic, Tris-hydrochloride, sodium chloride |
