8X8A
Crystal structure of STBD1 LIR motif in complex with GABARAPL1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-11-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 100.395, 100.395, 100.395 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.100 - 1.530 |
| R-factor | 0.1735 |
| Rwork | 0.173 |
| R-free | 0.18680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lxi |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.045 |
| Data reduction software | AutoProcess |
| Data scaling software | AutoProcess |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19rc4_4035) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.198 | 1.555 |
| High resolution limit [Å] | 1.529 | 1.529 |
| Rmerge | 0.098 | |
| Rpim | 0.370 | |
| Number of reflections | 25598 | 25582 |
| <I/σ(I)> | 24.9 | |
| Completeness [%] | 100.0 | |
| Redundancy | 38.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 25% w/v Pentaerythritol Propoxylate (5/4 PO/OH), 0.1 M Sodium acetate pH 4.6, 0.05 M Magnesium chloride |
