8X7X

Crystal structure of SADS-CoV fusion core

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	80
Detector technology	CCD
Collection date	2023-04-01
Detector	MAR CCD 165 mm
Wavelength(s)	0.9785
Spacegroup name	H 3 2
Unit cell lengths	45.176, 45.176, 418.012
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.560 - 2.590
R-factor	0.2375
Rwork	0.235
R-free	0.28360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.779
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.560	2.680
High resolution limit [Å]	2.590	2.590
Number of reflections	5590	55
<I/σ(I)>	2
Completeness [%]	98.9
Redundancy	1
CC(1/2)	0.984	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	0.1M HEPES pH 7.5, 42% PEG 200