8X77
Enterovirus proteinase with host factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17B1 |
| Synchrotron site | SSRF |
| Beamline | BL17B1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-03 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 148.954, 57.479, 198.622 |
| Unit cell angles | 90.00, 110.88, 90.00 |
Refinement procedure
| Resolution | 19.990 - 3.520 |
| R-factor | 0.2762 |
| Rwork | 0.274 |
| R-free | 0.29140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.340 | 3.510 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 2.090 | |
| Number of reflections | 76091 | 11533 |
| <I/σ(I)> | 2.29 | |
| Completeness [%] | 97.9 | |
| Redundancy | 3.51 | |
| CC(1/2) | 0.930 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 296 | 0.02M Citric acid, 0.08M BIS-TRIS propane, pH8.8, 16% PEG 3350 |
