8X65
Crystal structure of X11P(P71T) xylanase from a metagenome derived gene from sugarcane bagasse collection site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2020-07-24 |
| Detector | BRUKER PHOTON 100 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.466, 68.547, 74.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.730 - 1.700 |
| R-factor | 0.1444 |
| Rwork | 0.143 |
| R-free | 0.16560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.229 |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.730 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.044 | 0.136 |
| Rmeas | 0.049 | 0.163 |
| Rpim | 0.020 | 0.089 |
| Number of reflections | 19998 | 1008 |
| <I/σ(I)> | 24 | 8.9 |
| Completeness [%] | 99.5 | 97.1 |
| Redundancy | 5.2 | 3.1 |
| CC(1/2) | 0.999 | 0.965 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.5 | 298 | 0.1 M MES monohydrate pH 6.5 12% w/v Polyethylene glycol 20,000 |
