8X5V
BlCas9-sgRNA-target DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 144.750, 99.210, 119.300 |
| Unit cell angles | 90.00, 97.09, 90.00 |
Refinement procedure
| Resolution | 118.389 - 2.000 |
| Rwork | 0.198 |
| R-free | 0.23110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.615 |
| Data reduction software | DIALS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 118.389 | 50.000 | 2.120 |
| High resolution limit [Å] | 2.000 | 6.000 | 2.000 |
| Rmerge | 0.250 | 0.076 | 4.111 |
| Rmeas | 0.078 | 4.186 | |
| Number of reflections | 112881 | 4301 | 18007 |
| <I/σ(I)> | 19.01 | ||
| Completeness [%] | 99.9 | ||
| Redundancy | 28 | ||
| CC(1/2) | 0.999 | 0.649 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 200 mM sodium-acetate pH 4.5, 15%-20% PEG 500 MME, 200 mM ammonium sulfate, 10 mM strontium chloride |
