8X5T
Crystal structure of Thermus thermophilus peptidyl-tRNA hydrolase in complex with adenosine 5'-monophosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2012-11-07 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 120.315, 120.315, 185.981 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 104.200 - 1.600 |
| R-factor | 0.14533 |
| Rwork | 0.144 |
| R-free | 0.16756 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.908 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 104.200 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.056 | 0.214 |
| Number of reflections | 104619 | 5177 |
| <I/σ(I)> | 83.5 | 20.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 21.2 | 20.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.9 M K/Na tartrate, 0.1 M MES buffer pH5.8, and 40 mM adenosine 5'-monophosphate |
