8X3R

Crystal structure of human WDR5 in complex with WDR5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	48.560, 52.660, 123.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.210 - 1.760
R-factor	0.1674
Rwork	0.165
R-free	0.20480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond angle	7.681
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.210	1.790
High resolution limit [Å]	1.760	1.760
Rmerge	0.045
Number of reflections	30780	1719
<I/σ(I)>	10.9
Completeness [%]	96.9
Redundancy	1.8
CC(1/2)	0.998	0.674

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.1 M magnesium formate dihydrate, 15% w/v polyethylene glycol 3350