8X1A

Crystal structure of periplasmic G6P binding protein VcA0625

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RRCAT INDUS-2 BEAMLINE PX-BL21
Synchrotron site	RRCAT INDUS-2
Beamline	PX-BL21
Temperature [K]	294.15
Detector technology	IMAGE PLATE
Collection date	2018-05-24
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	57.288, 63.312, 75.323
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.470 - 1.604
R-factor	0.1649
Rwork	0.163
R-free	0.19630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.410
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.600	1.630
High resolution limit [Å]	1.600	1.600
Rmerge		0.344
Number of reflections	36010	1545
<I/σ(I)>	19.8
Completeness [%]	98.7
Redundancy	4.6
CC(1/2)	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	294.15	PEG 400, phosphate-citrate