8WZZ

Short-chain dehydrogenase/reductase16 (SDR16) from Antrodia camphorata

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	57.979, 69.115, 76.772
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.450 - 1.950
R-factor	0.196
Rwork	0.192
R-free	0.23690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.845
Refinement software	PHENIX ((1.16_3549: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.450	2.000
High resolution limit [Å]	1.950	1.950
Rmerge	0.058	0.460
Number of reflections	23105	1589
<I/σ(I)>	13.3
Completeness [%]	100.0
Redundancy	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	30% (w/v) PEG 5000 MME, 100mM MES/ Sodium hydroxide pH 6.5, 200mM Ammonium sulfate