8WZO
Parkin in complex with phospho NEDD8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-09-28 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.8731 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 77.976, 47.747, 127.859 |
| Unit cell angles | 90.00, 105.38, 90.00 |
Refinement procedure
| Resolution | 40.947 - 2.250 |
| Rwork | 0.207 |
| R-free | 0.24850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5n2w |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.026 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.520 | 2.320 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 21772 | 1974 |
| <I/σ(I)> | 8.4 | |
| Completeness [%] | 99.8 | |
| Redundancy | 4.3 | |
| CC(1/2) | 0.993 | 0.643 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 0.02 M Sodium/potassium phosphate, 0.1 M Bis-Tris propane, 7.5, 20% w/v PEG 3350 |
