8WXX
Structure of WDR5 in complex with WIN motif containing SET1B E1750R/G1751V
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9779 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 115.801, 47.244, 129.675 |
| Unit cell angles | 90.00, 112.97, 90.00 |
Refinement procedure
| Resolution | 27.210 - 2.100 |
| R-factor | 0.1772 |
| Rwork | 0.174 |
| R-free | 0.21160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.127 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.095 | 0.474 |
| Number of reflections | 37965 | 1855 |
| <I/σ(I)> | 13.35 | |
| Completeness [%] | 99.2 | |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 0.1 M sodium citrate, pH 5.5, 16% PEG 4000, 10% 2-propanol |
