8WXO

Crystal structure of substrate-binding protein from Rhodothermus marinus (Dose III)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2017-12-28
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	C 1 2 1
Unit cell lengths	63.341, 64.122, 69.757
Unit cell angles	90.00, 96.08, 90.00

Refinement procedure

Resolution	39.080 - 1.680
R-factor	0.2201
Rwork	0.218
R-free	0.25650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.454
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.090	1.740
High resolution limit [Å]	1.680	1.680
Number of reflections	61756	3159
<I/σ(I)>	6.4
Completeness [%]	99.8
Redundancy	6
CC(1/2)	0.996	0.608

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	Tris-HCl, MgCl2, polyethylene glycol 3350