8WXM

Crystal structure of substrate-binding protein from Rhodothermus marinus (Dose I)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2017-12-28
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	C 1 2 1
Unit cell lengths	63.577, 63.925, 69.698
Unit cell angles	90.00, 96.04, 90.00

Refinement procedure

Resolution	39.070 - 1.500
R-factor	0.2207
Rwork	0.220
R-free	0.24670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.980
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.090	1.550
High resolution limit [Å]	1.500	1.500
Number of reflections	85769	4052
<I/σ(I)>	7.7
Completeness [%]	98.8	91.6
Redundancy	5.8	4.5
CC(1/2)	0.997	0.587

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	Tris-HCl, MgCl2, polyethylene glycol 3350