8WWO

Crystal structure of the AFSV topoisomerase ATPase domain in complex with AMPPNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 31
Unit cell lengths	85.658, 85.658, 212.071
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.960 - 2.200
R-factor	0.1779
Rwork	0.176
R-free	0.22090
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.217
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	87831	8623
<I/σ(I)>	11.1
Completeness [%]	96.5
Redundancy	3
CC(1/2)	0.994	0.731

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	0.2M Ammonium sulfate, 0.1M HEPES pH 7.5, 22.5% PEG 3350