8WVT

Crystal structure of Lsd18 in complex with ligands

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97915
Spacegroup name	P 1
Unit cell lengths	46.921, 61.804, 75.633
Unit cell angles	74.61, 81.74, 76.79

Refinement procedure

Resolution	25.284 - 1.750
R-factor	0.2105
Rwork	0.208
R-free	0.24880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8up4
RMSD bond length	0.007
RMSD bond angle	0.855
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.16_3549: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.284	1.813
High resolution limit [Å]	1.750	1.750
Rmerge	0.145	0.437
Rmeas	0.206	0.293
Rpim	0.145	0.528
Number of reflections	76354	3815
<I/σ(I)>	8.5	4.1
Completeness [%]	95.3	86.63
Redundancy	1
CC(1/2)	0.885	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	0.1 M Tris pH7.5, 1.0 M NaCl, 32% (w/v) PEG8000, 4% tert-butanol