8WV8

Crystal Structure of Cyanobacterial Circadian Clock Protein KaiC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9
Spacegroup name	P 63
Unit cell lengths	95.191, 95.191, 184.086
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.220 - 2.930
R-factor	0.2783
Rwork	0.276
R-free	0.33510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	1.234
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.220	3.030
High resolution limit [Å]	2.930	2.930
Rmerge	0.117
Number of reflections	20257	1985
<I/σ(I)>	15.8
Completeness [%]	99.7
Redundancy	10.3
CC(1/2)		0.913

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7	313	sodium/pottasium tartrae, sodium acetate, KCl