8WUG
The Crystal Structure of JMJD2D from Biortus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-26 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.978530 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 71.340, 71.340, 150.873 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.887 - 1.700 |
| Rwork | 0.161 |
| R-free | 0.18760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.131 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.890 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.029 | 0.162 |
| Number of reflections | 40301 | 2235 |
| <I/σ(I)> | 60.6 | 13.4 |
| Completeness [%] | 92.0 | |
| Redundancy | 15.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M HEPES, NaOH pH 7.5, 2% PEG 400, 2M Ammonium Sulfate |
