8WTK

Crystal structure of Cas3-DExD+MG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	293
Detector technology	CCD
Collection date	2020-01-01
Detector	MAR CCD 165 mm
Wavelength(s)	1.0000
Spacegroup name	P 31 2 1
Unit cell lengths	114.756, 114.756, 119.632
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.980 - 3.080
R-factor	0.2286
Rwork	0.224
R-free	0.26950
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.603
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.000	3.190
High resolution limit [Å]	3.080	3.080
Number of reflections	17162	1668
<I/σ(I)>	6
Completeness [%]	99.5
Redundancy	5
CC(1/2)	0.850	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		295.15	15% PEG3350, 10% glycerol, and 0.1 M Tris at a pH of 6.4