8WS0
Crystal structure of human NEK7 S195D mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-28 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.953643918037 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 88.601, 88.601, 156.088 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.340 - 2.120 |
| R-factor | 0.1936 |
| Rwork | 0.191 |
| R-free | 0.24176 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.917 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.340 | 2.180 |
| High resolution limit [Å] | 2.117 | 2.117 |
| Rmerge | 0.058 | 1.149 |
| Number of reflections | 41004 | 3084 |
| <I/σ(I)> | 28.5 | |
| Completeness [%] | 99.5 | 93.6 |
| Redundancy | 20 | |
| CC(1/2) | 1.000 | 0.810 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.1 M Tris pH 8.5, 20% PEG 3350 |
