8WQQ

Proteomics study reveals that ASFV g5Rp protein interacts with eukaryotic translation initiation factor 5A and may regulate host translation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2022-03-05
Detector	AGILENT ATLAS CCD
Wavelength(s)	0.97931
Spacegroup name	P 21 21 21
Unit cell lengths	74.199, 77.296, 96.779
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.198 - 2.300
R-factor	0.2415
Rwork	0.237
R-free	0.28730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.947
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3247: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.198	2.490
High resolution limit [Å]	2.300	2.400
Rmerge	0.115	0.778
Number of reflections	25174	1627
<I/σ(I)>	15.4
Completeness [%]	99.3
Redundancy	12.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	0.1M HEPES Sodium, pH 7.5,1.5M Lithium Sulfate monophydrate