8WQJ
Crystal Structure of Transaminase from Shimia marina
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-05-31 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.97949 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.710, 73.440, 184.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.610 - 2.140 |
R-factor | 0.1778 |
Rwork | 0.176 |
R-free | 0.21590 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.900 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.20_4459: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.610 | 2.216 |
High resolution limit [Å] | 2.140 | 2.140 |
Number of reflections | 49439 | 4867 |
<I/σ(I)> | 10.95 | |
Completeness [%] | 99.8 | |
Redundancy | 9 | |
CC(1/2) | 0.994 | 0.763 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M BIS-TRIS pH 6.5 25% w/v Polyethylene glycol 3350 |