8WQ5

Crystal structure of the C-terminal RRM domain of an RBP in complex with ssDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	P 1 21 1
Unit cell lengths	42.403, 83.767, 77.762
Unit cell angles	90.00, 96.87, 90.00

Refinement procedure

Resolution	38.970 - 1.650
R-factor	0.1608
Rwork	0.158
R-free	0.20690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.741
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.710
High resolution limit [Å]	1.650	3.550	1.650
Rmerge	0.121	0.066	0.974
Rmeas	0.136	0.075	1.084
Rpim	0.060	0.034	0.468
Total number of observations	327484
Number of reflections	64857	6603	6460
<I/σ(I)>	7.5
Completeness [%]	99.8	99.8	99.8
Redundancy	5	4.8	5.1
CC(1/2)	0.998	0.995	0.841

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.3	289	8% PEG 2000 and 0.1M succinic acid, pH 5.3