8WQ5
Crystal structure of the C-terminal RRM domain of an RBP in complex with ssDNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-08-22 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.403, 83.767, 77.762 |
| Unit cell angles | 90.00, 96.87, 90.00 |
Refinement procedure
| Resolution | 38.970 - 1.650 |
| R-factor | 0.1608 |
| Rwork | 0.158 |
| R-free | 0.20690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.741 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 3.550 | 1.650 |
| Rmerge | 0.121 | 0.066 | 0.974 |
| Rmeas | 0.136 | 0.075 | 1.084 |
| Rpim | 0.060 | 0.034 | 0.468 |
| Total number of observations | 327484 | ||
| Number of reflections | 64857 | 6603 | 6460 |
| <I/σ(I)> | 7.5 | ||
| Completeness [%] | 99.8 | 99.8 | 99.8 |
| Redundancy | 5 | 4.8 | 5.1 |
| CC(1/2) | 0.998 | 0.995 | 0.841 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | 289 | 8% PEG 2000 and 0.1M succinic acid, pH 5.3 |
