8WOP
Crystal structure of Arabidopsis thaliana UDP-glucose 4-epimerase 2 (AtUGE2) complexed with UDP, wild-type
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-13 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.309, 110.847, 65.552 |
| Unit cell angles | 90.00, 106.14, 90.00 |
Refinement procedure
| Resolution | 48.230 - 2.350 |
| R-factor | 0.18041 |
| Rwork | 0.178 |
| R-free | 0.23591 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ek6 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.456 |
| Data reduction software | XDS (Feb 5, 2021) |
| Data scaling software | XDS (Feb 5, 2021) |
| Phasing software | MOLREP (11.0) |
| Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.230 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.098 | 0.569 |
| Rpim | 0.062 | 0.374 |
| Number of reflections | 28665 | 2785 |
| <I/σ(I)> | 9.2 | 3.2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.4 | 3.2 |
| CC(1/2) | 0.995 | 0.728 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 20-22% (w/v) PEG3350 and 0.16-0.18 M magnesium formate |
