8WO8
Crystal Structure of an RNA-binding protein, FAU-1, from Pyrococcus furiosus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-17 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 119.573, 119.573, 93.472 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.010 - 2.780 |
| R-factor | 0.20985 |
| Rwork | 0.206 |
| R-free | 0.26797 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AF-Q8U4Q7-F1-model_v4 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.733 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.780 | 2.670 |
| High resolution limit [Å] | 2.580 | 2.670 | 2.580 |
| Rmerge | 0.121 | 0.937 | |
| Number of reflections | 22452 | 1704 | 937 |
| <I/σ(I)> | 12 | 1.17 | 0.48 |
| Completeness [%] | 90.2 | 93.5 | 38.1 |
| Redundancy | 9.4 | 5.7 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 40%(v/v) PEG-300, 0.1 mM HEPES (pH 7.5), 0.2 M NaCl |
