8WME
Crystal Structure of Drosophila melanogaster D46A Dopamine N-Acetyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-04-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.99984 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.663, 66.952, 84.464 |
| Unit cell angles | 90.00, 102.46, 90.00 |
Refinement procedure
| Resolution | 28.776 - 2.283 |
| R-factor | 0.1912 |
| Rwork | 0.188 |
| R-free | 0.25310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP (6.5) |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.280 |
| Rmerge | 0.116 |
| Rmeas | 0.128 |
| Rpim | 0.054 |
| Number of reflections | 18954 |
| <I/σ(I)> | 13.46 |
| Completeness [%] | 96.1 |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 1.0M NaH2PO4, 1.6M K2HPO4, 0.1M HEPES, 0.2M NaCl |
