8WLG
Crystal structure of Cypovirus Polyhedra mutant fused with c-Myc fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-04-26 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1 |
Spacegroup name | I 2 3 |
Unit cell lengths | 105.140, 105.140, 105.140 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.170 - 2.550 |
R-factor | 0.2214 |
Rwork | 0.216 |
R-free | 0.26950 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.986 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.700 |
High resolution limit [Å] | 2.550 | 2.550 |
Number of reflections | 6468 | 1003 |
<I/σ(I)> | 11.44 | |
Completeness [%] | 100.0 | |
Redundancy | 78.8 | |
CC(1/2) | 0.990 | 0.632 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 293 | cell-free crystallization |