8WIM
Crystal structure of Jingmen tick virus RNA-dependent RNA polymerase (D307 construct)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-12-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.522, 89.251, 122.405 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.110 - 1.840 |
| R-factor | 0.1818 |
| Rwork | 0.180 |
| R-free | 0.20920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | in-house model determined by SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.918 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.12) |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.910 |
| High resolution limit [Å] | 1.840 | 3.960 | 1.840 |
| Rmerge | 0.077 | 0.036 | 0.583 |
| Rmeas | 0.083 | 0.039 | 0.671 |
| Rpim | 0.031 | 0.014 | 0.320 |
| Total number of observations | 395284 | ||
| Number of reflections | 58904 | 6204 | 5474 |
| <I/σ(I)> | 9.6 | ||
| Completeness [%] | 99.1 | 98.9 | 93.5 |
| Redundancy | 6.7 | 7.1 | 3.6 |
| CC(1/2) | 0.999 | 0.999 | 0.772 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 289 | potassium sodium tartrate tetrahyrate |
