8WHJ

Crystal structure of CLASP2 TOG4 fused with LL5beta

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.98
Spacegroup name	P 41
Unit cell lengths	51.132, 51.132, 97.935
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.566 - 1.400
R-factor	0.1311
Rwork	0.129
R-free	0.17350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.172
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.420
High resolution limit [Å]	1.400	3.800	1.400
Rmerge	0.064	0.036	0.822
Rmeas	0.067	0.037	0.866
Rpim	0.019	0.010	0.267
Number of reflections	49279	2524	2432
<I/σ(I)>	7.2
Completeness [%]	99.8	99.9	99.8
Redundancy	11.7	12.2	9.7
CC(1/2)	1.000	0.999	0.890

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2 M Sodium chloride, 0.1 M Bis-Tris pH 5.5, and 25% w/v PEG 3350