8WG0

Crystal structure of GH97 glucodextranase from Flavobacterium johnsoniae in complex with glucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-05
Detector	DECTRIS PILATUS3 S 2M
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	307.927, 103.691, 95.671
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.130 - 1.950
Rwork	0.189
R-free	0.22890
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.902
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0405)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.130	1.980
High resolution limit [Å]	1.950	1.950
Rmerge	0.090	1.471
Rmeas	0.097	1.588
Rpim	0.037	0.594
Number of reflections	223030	10951
<I/σ(I)>	23	2.2
Completeness [%]	100.0	99.9
Redundancy	13.5	13.6
CC(1/2)	1.000	0.836

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	12% PEG20000, 200 mM sodium cacodylate buffer (pH 6.0), 200 mM magnesium acetate, 10 mM glucose