8WFV

Crystal structure of beta-glucosidase from Thermoanaerobacterium saccharolyticum (Data 3)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9794
Spacegroup name	P 21 21 21
Unit cell lengths	64.970, 70.948, 98.876
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.440 - 1.500
R-factor	0.155
Rwork	0.155
R-free	0.17120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.648
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.440	1.530
High resolution limit [Å]	1.500	1.500
Number of reflections	71920	3026
<I/σ(I)>	15.41
Completeness [%]	97.4
Redundancy	4.1
CC(1/2)	0.973	0.771

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	Tris-HCl, PEG 4000, MgCl2