8WFR

The Crystal Structure of PCSK9 from Biortus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	63.081, 70.813, 149.867
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.260 - 1.950
Rwork	0.169
R-free	0.20340
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.017
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.260	2.000
High resolution limit [Å]	1.950	1.950
Number of reflections	49827	3453
<I/σ(I)>	11.4
Completeness [%]	100.0
Redundancy	7.4
CC(1/2)	0.996	0.707

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M HEPES pH7.5, 20% PEG 10000