8WFQ

The Crystal Structure of RALDH1 from Biortus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-21
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.95374
Spacegroup name	P 1 21 1
Unit cell lengths	101.343, 149.560, 125.076
Unit cell angles	90.00, 95.43, 90.00

Refinement procedure

Resolution	48.160 - 3.500
Rwork	0.230
R-free	0.25430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.667
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.160	3.620
High resolution limit [Å]	3.500	3.500
Number of reflections	46102	4537
<I/σ(I)>	4.7
Completeness [%]	98.5
Redundancy	3
CC(1/2)	0.950	0.618

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M CaCl2, 0.1M MgCl2, 0.1M PIPES pH7, 22.5% PEG smear