8WFF

The Crystal Structure of LYN from Biortus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.99999
Spacegroup name	P 61
Unit cell lengths	45.730, 45.730, 56.686
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.603 - 1.300
Rwork	0.155
R-free	0.18220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.569
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.603	1.320
High resolution limit [Å]	1.300	1.300
Rmerge	0.078	0.293
Number of reflections	16562	790
<I/σ(I)>	19.2
Completeness [%]	99.7
Redundancy	9.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M Citric acid pH3.5, 3.0M NaCl