8WFC

N5, N10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	80
Detector technology	PIXEL
Collection date	2023-05-03
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98
Spacegroup name	P 43 2 2
Unit cell lengths	110.681, 110.681, 69.781
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.000 - 2.050
R-factor	0.2161
Rwork	0.215
R-free	0.23780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.131
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MOLREP
Refinement software	REFMAC (5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.090
High resolution limit [Å]	2.050	2.050
Rmerge	0.100	0.304
Number of reflections	27821	1347
<I/σ(I)>	59.67
Completeness [%]	99.9	99.9
Redundancy	26

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		296	Sodium Potassium phosphate, MPD