8WEH

Crystal structure of Brassica napus MIK2 ectodomain (N393A mutant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2023-04-10
Detector	PSI PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 31 2 1
Unit cell lengths	156.845, 156.845, 125.569
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.720 - 3.600
R-factor	0.2018
Rwork	0.201
R-free	0.21060
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.571
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.730
High resolution limit [Å]	3.600	3.600
Number of reflections	24972	2021
<I/σ(I)>	5.4
Completeness [%]	97.8
Redundancy	2.9
CC(1/2)	0.950	0.518

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8.5	289	0.2 M Lithium sulfate, 0.1 M Tris pH 8.5, 34% w/v PEG 400