8WEE

Crystal structure of Arabidopsis thaliana MIK2 ectodomain in complex with SCOOP12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2022-09-09
Detector	PSI PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 21 21 21
Unit cell lengths	61.803, 108.135, 164.145
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.190 - 2.190
R-factor	0.1767
Rwork	0.176
R-free	0.19910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.176
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.190	2.190
Number of reflections	56428	5279
<I/σ(I)>	13.9
Completeness [%]	98.4
Redundancy	5.2
CC(1/2)	0.980	0.920

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.4	289	0.2 M Sodium acetate, 0.1 M Tris pH 7.4 and 18% (w/v) PEG 3350